Selection of Elite Maintainers with eui Gene using RAPD Analysis for Development of New eMS Lines in Hybrid Rice Breeding

Abstract

Random Amplified Polymorphic DNA (RAPD) analysis was employed for genetic diversity study over 32 rice
genotypes including 29 agronomically superior maintainer lines with eui gene for good panicle exertion and 3
restorers. A total of 102 amplification products were detected with 12 polymorphic RAPD primers of which 69
(67.64%) were polymorphic. Polymorphic primer showed 50 to 80 amplicon polymorphism. The genetic similarity
ranging between 54.2% (L I9-Ls) to 92% (L11-L2) suggested that the maintainers and restorers under study are
divergent. Cluster generated through UPGMAanalysis revealed two main clusters. Cluster I comprised two genotypes
only, both derived from the same cross. Genotypes in cluster II were redistributed into a number of subclusters
at different similarity coefficient levels indicating different levels of genetic relationships. The maintainer lines
L2, L14' L17, L20 and L29 used in the study are suggested to be exploited for development of CMS lines to realize
higher level of heterosis. The hybrids involving parents with maximum dissimilarity coefficients displayed nonsignificant
estimates of heterosis for grain yield